Radioimmunoassay of succinyl cyclic CMP and measurement of cyclic CMP in rat tissues.
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چکیده
منابع مشابه
Cyclic CMP and cyclic UMP: new (old) second messengers
Historical background As early as 1965 it was proposed that in addition to the well-established second messengers, cyclic AMP and cyclic GMP, the cyclic pyrimidine nucleotides, cyclic CMP (cCMP) and cyclic UMP (cUMP) could play a role as second messenger molecules. This hypothesis was based on the identification of cCMPand cUMP-hydrolyzing phosphodiesterase (PDE) activities in mammalian tissues...
متن کاملPurification to homogeneity and general properties of a novel phosphodiesterase hydrolyzing cyclic CMP and cyclic AMP.
A cytidine 3’5’-monophosphate (cyclic CMP) phosphodiesterase was purified more than 10,000-fold to apparent homogeneity from the pig liver extract by the steps of pH and ammonium sulfate fractionations, and DEAE-cellulose, Sephadex G-100, and 8-HzN(CH&NHcyclic AMP Sepharose 4B affinity chromatographies. The preparation appeared as a single protein band in sodium dodecyl sulfate-polyacrylamide g...
متن کاملBinding of Regulatory Subunits of Cyclic AMP-Dependent Protein Kinase to Cyclic CMP Agarose
The bacterial adenylyl cyclase toxins CyaA from Bordetella pertussis and edema factor from Bacillus anthracis as well as soluble guanylyl cyclase α(1)β(1) synthesize the cyclic pyrimidine nucleotide cCMP. These data raise the question to which effector proteins cCMP binds. Recently, we reported that cCMP activates the regulatory subunits RIα and RIIα of cAMP-dependent protein kinase. In this st...
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A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and...
متن کاملThe Measurement of Cyclic Adenylate in Tissues.
I Harvey, W. R., and C. M. Williams, J. Insect Physiol., 7, 81 (1961). 3 Scherrer, K., and J. E. Darnell, Biochem. Biophys. Res. Commun., 7, 486 (1962). 4Wyatt, G. R., in Insect Physiology, ed. V. Brookes (Oregon State University Press, 1963), p. 23. 5 Britten, R. J., and R. B. Roberts, Science, 131, 32 (1960). 6 The specific activities for the incorporation of uridine-2-C14 and uridine-H3 can ...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1980
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)53605-8